Properties of Guaiacol Peroxidase Activities Isolated from Corn Root Plasma Membranes

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Properties of guaiacol peroxidase activities isolated from corn root plasma membranes.

Although several investigations have demonstrated a plasma membrane (PM)-bound peroxidase activity in plants, this study is the first, to our knowledge, to purify and characterize the enzymes responsible. Proteins were extracted from highly enriched and thoroughly washed PMs. Washing and solubilization procedures indicated that the enzymes were tightly bound to the membrane. At least two distin...

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Labeling and isolation of plasma membranes from corn leaf protoplasts.

A plasma membrane-enriched fraction has been isolated from corn leaf mesophyll protoplasts and its identity confirmed with the aid of an external label, diazotized [(125)I]iodosulfanilic acid. Gentle cell disruption enabled internal organelles to be maintained intact and thus facilitated separation from the plasma membrane. The plasma membrane-enriched fraction was devoid of chloroplast or mito...

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Kinetics Properties of Guaiacol Peroxidase Activity in Crocus sativus L. Corm during Rooting

Guaiacol peroxidases (GP) are haem-containing enzymes participating in many physiological processes in plants. The expression pattern of these enzymes is organ-specific and developmentally regulated. Methods: The presence of GP activity in extract samples, prepared from Crocus sativus L. corms that were either dormant or rooting for 3, 6 and 10 days, was investigated. Results: Kinetic studies r...

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ژورنال

عنوان ژورنال: Plant Physiology

سال: 2003

ISSN: 1532-2548,0032-0889

DOI: 10.1104/pp.103.020396